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six well polystyrene tissue culture treated plates  (CELLTREAT Scientific)


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    CELLTREAT Scientific six well polystyrene tissue culture treated plates
    Six Well Polystyrene Tissue Culture Treated Plates, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/six+well+polystyrene+tissue+culture+treated+plates/pm40635271-394-22-32?v=CELLTREAT+Scientific
    Average 95 stars, based on 33 article reviews
    six well polystyrene tissue culture treated plates - by Bioz Stars, 2026-08
    95/100 stars

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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
    Six Well Tissue Culture Treated Polystyrene Plate With A Transwell Insert (0.4 μm Polyester Membrane), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
    Tissue Culture Treated Polystyrene Six Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson tissue culture treated polystyrene six-well plates
    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
    Tissue Culture Treated Polystyrene Six Well Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/six+well+polystyrene+tissue+culture+treated+plates/pm24010580-68-8-10?v=Becton+Dickinson
    Average 90 stars, based on 1 article reviews
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
    Six Well Tissue Culture Treated Polystyrene Plate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/six+well+polystyrene+tissue+culture+treated+plates/pm24667769-102-21-26?v=Fisher+Scientific
    Average 90 stars, based on 1 article reviews
    six-well tissue-culture treated polystyrene plate - by Bioz Stars, 2026-08
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    Fisher Scientific six-well tissue culture-treated polystyrene plates
    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a <t>Transwell</t> membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.
    Six Well Tissue Culture Treated Polystyrene Plates, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/six+well+polystyrene+tissue+culture+treated+plates/pm23795974-50-14-16?v=Fisher+Scientific
    Average 90 stars, based on 1 article reviews
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    L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a Transwell membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.

    Journal: Journal of Bacteriology

    Article Title: Genome-wide analysis of Enterococcus faecalis genes that facilitate interspecies competition with Lactobacillus crispatus

    doi: 10.1128/jb.00438-24

    Figure Lengend Snippet: L. crispatus antagonism of E. faecalis is facilitated in a contact-independent manner. ( A ) CFU recovered from E. faecalis OG1RF and L. crispatus VPI 3199 grown statically for 72 h in MRS media as single-species biofilm, either in separate wells or in the same well separated by a Transwell membrane insert that prevents physical contact between cells. L. crispatus biofilm is grown on the flat surface of the well in the tissue culture plate, whereas E. faecalis biofilm is seeded on the surface of the Transwell membrane insert. The dotted line indicates the limit of detection (LOD), CFU < 42.5. ( B ) Representative images of spot antagonism assay showing growth inhibition of E. faecalis when L. crispatus macrocolony biofilms were established at the same time (T0), 24 h, 48 h, or 72 h before inoculating E. faecalis . CFU recovered from E. faecalis OG1RF growth after 24 h in MRS media mixed with 72 h cell-free biofilm supernatant isolated from single-species and dual-species biofilms at an equal ratio, either ( C ) pH-unadjusted or ( D ) adjusted to pH 6.5 to mirror the MRS media. For A, C, and D, data points represent 9–12 biological replicates, collated from at least three repeated experiments. Statistical analysis was performed using the Brown-Forsythe ANOVA test with Welch’s correction. Error bars represent the standard error of the mean. ** P ≤ 0.01, **** P ≤ 0.0001.

    Article Snippet: To investigate whether L. crispatus -mediated killing was contact-dependent or independent, biofilms were allowed to form in a six-well tissue culture-treated polystyrene plate with a Transwell insert (0.4 μm polyester membrane; Lot #20623011) (Corning, New York, USA).

    Techniques: Membrane, Inhibition, Isolation